human recombinant c-src enzyme (Millipore)

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Millipore human recombinant c-src enzyme
Human Recombinant C Src Enzyme, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+src+enzyme/recombinant+human+c+src/pm09516477-45-2-9
Average 90 stars, based on 1 article reviews

human recombinant c-src enzyme - by Bioz Stars, 2026-10

90/100 stars

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Enzyme-linked Immunosorbent Assay:

Article Title: Synergy among non-desmoglein antibodies contributes to the immunopathology of desmoglein antibody–negative pemphigus vulgaris
Article Snippet: .. The ELISA kits for measuring phosphorylated Src was purchased from EMD Biosciences, Inc. (La Jolla, CA). .. The enzymatic activity of Cs9, and concentrations of CytC and phosphorylated p38 were measured using kits purchased from R&D Systems, Inc. (Minneapolis, MN).

Article Title: Gα 13 Switch Region 2 Binds to the Talin Head Domain and Activates αIIbβ3 Integrin in Human Platelets
Article Snippet: .. Reagents were from the following sources: ADP and dimethyl-BAPTA-AM (Invitrogen); U46619 (Cayman Chemical); polyclonal rabbit anti-kindlin-3 and the monoclonal anti-αIIb, anti-β3, whole talin antibodies, and fibronectin (Abcam); HRP-conjugated goat anti-rabbit antibody (Cell Signaling); BCA protein assay kit and nitrocellulose membranes (Bio-Rad), Pierce Supersignal kit, TMB and ECL chemiluminescent substrates (Pierce Biochemicals); Streptavidin-HRP (Life Technologies); nitrocellulose blotting membranes, pGEX6p2, and glutathione-Sepharose 4B resin (GE Life Sciences); IPTG and nickel metal affinity chromatography (GoldBio); Src ELISA activation assay kit (Millipore); RhoA G-LISA TM activation assay kit and cell lysis buffer (Cytoskeleton); SulfoLink immoblization kit for peptides and the FITC-PAC1 antibody (Thermo Fisher Scientific); PAC1 monoclonal antibody (Biolegend); protein A-Sepharose beads (Sigma-Aldrich); trypsin EDTA (Corning); Rap1 antibody (Bethyl Laboratories); Gα13, His-probe, and GST antibody (Santa Cruz Biotechnology); GFP-C1 plasmid (Clontech Laboratories, Inc); Immulon 2 Removawells (Dynatech Laboratories, Inc). ..

Article Title: Recruitment of calcineurin to the T cell receptor positively regulates T cell activation
Article Snippet: Recombinant human calcineurin (catalog no. 3160-CA, R&D Systems), Lipofectamine 2000 (Life Technologies), calmodulin from bovine testes (catalog no. P1431, Sigma), piceatannol (10009366, Cayman Chemical); 11R-VIVIT (480401) and 11R-VEET (catalog no. 480401, Calbiochem; moth cytochrome c (MCC) peptide (ANERADLIAYLKQATK; Peptide 2.0), recombinant human ICAM1–CD54-Fc chimera (720-IC-050, R&D Systems); human CD4 and mouse T cell recovery column kits (Cedarlane Laboratories Limited), and control siRNA-A (sc-37007), PP2B-Aα siRNA (sc-36304) and PP2B-Aß siRNA (sc-39195) (all from Santa Cruz) were used. .. FK506 monohydrate and cyclosporin A were from Sigma; the Src kinase inhibitor PP1 was from Calbiochem; and the human IL-2 ELISA kit was from eBioscience. .. SDS sample buffer was purchased from Quality Biologicals Inc., and Restore PLUS western blot Stripping Buffer was from Thermo Scientific.

Article Title: Synergy among non-desmoglein antibodies contributes to the immunopathology of desmoglein antibody–negative pemphigus vulgaris
Article Snippet: .. Keratinocyte cell culture experiments The ELISA kits for measuring phosphorylated Src was purchased from EMD Biosciences, Inc. (La Jolla, CA). .. The enzymatic activity of caspase 9, and concentrations of CytC and phosphorylated p38 were measured using kits purchased from R&D Systems, Inc. (Minneapolis, MN).

Article Title: Synergy among non-desmoglein antibodies contributes to the immunopathology of desmoglein antibody–negative pemphigus vulgaris
Article Snippet: .. Keratinocyte cell culture experiments The ELISA kits for measuring phosphorylated Src was purchased from EMD Biosciences, Inc. (La Jolla, CA). .. The enzymatic activity of Cs9, and concentrations of CytC and phosphorylated p38 were measured using kits purchased from R&D Systems, Inc. (Minneapolis, MN).

Bicinchoninic Acid Protein Assay:

Affinity Chromatography:

Activation Assay:

Lysis:

Plasmid Preparation:

other:

Article Title: Angiotensin II-induced association of phospholipase Cgamma1 with the G-protein-coupled AT1 receptor.
Article Snippet: Purified human recombinant c-Src enzyme and PP1 came from Calbiochem.

Article Title: An Important Role of the Src Family Kinase Lyn in Stimulating Platelet Granule Secretion
Article Snippet: Collagen and luciferase/luciferin reagent were from Chrono-log (Havertown, PA).

Cell Culture: